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Article Title: Interstrain Variation of the Polysaccharide B Biosynthesis Locus of Bacteroides fragilis : Characterization of the Region from Strain 638R

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Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Cosmid clones pLEC17, pLEC18, and pLEC19 were selected by using a probe consisting of an internal portion of orf7 of strain NCTC 9343, an open reading frame (ORF) found by PCR to be common to both strains (Fig. ). table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Description Reference and/or source E. coli strains DH5α F − φ80d lacZΔM15 Δ( lacZYA-argF ) U169 endA1 recA1 hsdR17 (r K − m K + ) deoR thi-1 supE44 gyrA96 relA1 λ − 1 AB1133 K-12 thr-1 leuB6 Δ( gpt-proA ) 66 hisG4 argE3 thi-1 rfbD1 lacY1 ara-14 galK2 xyl-5 mtl-1 mgl-51 rpsL31 kdgK51 supE44 7 21546 AB1133 with rff ::Tn 10 -46 7 21566 AB1133 with rff ::Tn 10 -66 7 B. fragilis strains NCTC 9343 Type strain, appendix abscess 5 , ATCC a 638R (TM4000) Clinical 12 2-42 638R PS B mutant with transposon insertion in wcg locus 2 Plasmids pHC79 Cosmid vector; Ap r 4 pUC19 Cloning vector; Ap r New England Biolabs pLEC6.1 900-bp Eco RI- Hin dIII fragment ( B. fragilis DNA) cloned in pBluescript; Ap r 2 pMJC10 638R gene bank clone including orf3-wcgE cloned in pHC79; Ap r This study pLEC17 638R gene bank clone including wcgM-orf7 cloned in pHC79; Ap r This study pLEC18 638R gene bank clone including wcgJ-orf7 cloned in pHC79; Ap r This study pLEC19 638R gene bank clone including wcgF-orf7 cloned in pHC79; Ap r This study pWCGC 1,424-bp PCR amplicon containing wcgC cloned in pUC19; Ap r This study pWCGD 1,518-bp PCR amplicon containing wcgD cloned in pUC19; Ap r This study pWCGCD 2,711-bp PCR amplicon containing wcgC-wcgD cloned in pUC19; Ap r This study Open in a separate window a ATCC, American Type Culture Collection.

Techniques: Plasmid Preparation, Mutagenesis, Clone Assay, Amplification